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Anti-CD200R Polyclonal Antibody

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Catalog No. TMAB-03911

Anti-CD200R Polyclonal Antibody is a Rabbit antibody targeting CD200R. Anti-CD200R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-CD200R Polyclonal Antibody

Anti-CD200R Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-03911
Anti-CD200R Polyclonal Antibody is a Rabbit antibody targeting CD200R. Anti-CD200R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD200R Polyclonal Antibody is a Rabbit antibody targeting CD200R. Anti-CD200R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated (TMAB-03911) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Sample: hela (Human) Cell Lysate at 30 μg
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-CD200R (TMAB-03911) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 58 kD
3. Blank control: Hepg2 Cells (blue). Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody (TMAB-03911-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted and Cell membrane.
Tissue SpecificityExpressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD200R
Antigen Species
Human
Gene Name
CD200R1
Gene ID
Protein Name
Cell surface glycoprotein CD200 receptor 1
Uniprot ID
Function
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Chemical Properties
Molecular WeightTheoretical: 34 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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