Anti-CD1d Polyclonal Antibody 3 is a Rabbit antibody targeting CD1d. Anti-CD1d Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Mouse,Rat

1. Sample:
Thymus (Rat) Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-CD1d (TMAB-03896) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 61 kD
2. Blank control: mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CD1A antibody (TMAB-03896), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol. Primary antibody (TMAB-03896, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
3. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-CD1d (TMAB-03896) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 61 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CD1D

Mouse

CD1D

Antigen-presenting glycoprotein CD1d

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.