Anti-CD1c Antibody (4N604) is a Rabbit antibody targeting CD1c. Anti-CD1c Antibody (4N604) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

4N604

Human

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: THP-1 (Negative control), 2: MOLT-4, 3: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 38 kDa
Observed MW: 43 kDa
2. Tissue: Human spleen
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03894
3. Tissue: Human tonsil
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03894

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human CD1c

Human

CD1C

T-cell surface glycoprotein CD1c

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.