Anti-CD1A Antibody (4F102) is a Rabbit antibody targeting CD1A. Anti-CD1A Antibody (4F102) can be used in IHC-P,FCM,IHC-Fr,IF,WB.

IgG

4F102

Human

1. Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD1A) Monoclonal Antibody, Unconjugated (TMAB-03891) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD1A Monoclonal Antibody, Unconjugated (TMAB-03891) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD1A Monoclonal Antibody, Unconjugated (TMAB-03891) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD1A Monoclonal Antibody, Unconjugated (TMAB-03891) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD1A Monoclonal Antibody, Unconjugated (TMAB-03891) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. The Molt-4 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD1a antibody (TMAB-03891): 1 μg/10^6 cells. Blank control (black): PBS. Acquisition of 20,000 events was performed.
7. 25 μg total protein per lane of various lysates (see on figure) probed with CD1a monoclonal antibody, unconjugated (TMAB-03891) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IHC-P: 1:100-500; FCM: 1μg/Test; IHC-Fr: 1:100-500; IF: 1:100-500; WB: 1:200-1000

Monoclonal

A synthesized peptide: human CD1a

Human

CD1A

T-cell surface glycoprotein CD1a

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.