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Anti-CD19 Antibody (5R664) is a Rabbit antibody targeting CD19. Anti-CD19 Antibody (5R664) can be used in ICC/IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
| Description | Anti-CD19 Antibody (5R664) is a Rabbit antibody targeting CD19. Anti-CD19 Antibody (5R664) can be used in ICC/IF,IHC,WB. |
| Synonyms | CVID3, CD19 molecule, B4 |
| Ig Type | IgG |
| Clone | 5R664 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of CD19 on Raji cells lysates using anti-CD19 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin. 4. ICC staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 95 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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