Anti-CD166 Polyclonal Antibody is a Rabbit antibody targeting CD166. Anti-CD166 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Blank control: Molt-4 Cells (blue). Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody (TMAB-03871/AF488), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 orange), used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (TMAB-03871/AF488, 1 μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
2. Sample:
MCF-7 Cell (Human) Lysate at 30 μg
Primary: Anti-CD166 (TMAB-03871) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
3. Sample:
HepG2 Cell (Human) Lysate at 30 μg
Primary: Anti-CD166 (TMAB-03871) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD166

Human

ALCAM

CD166 antigen

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and hemophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.