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Anti-CD16/FCGR3 Antibody (5V408)
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Catalog No. TMAY-01518
Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Antibody (5V408) is a Mouse antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (5V408) can be used in FCM.
Anti-CD16/FCGR3 Antibody (5V408)
Anti-CD16/FCGR3 Antibody (5V408)
😃Good
Catalog No. TMAY-01518Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Antibody (5V408) is a Mouse antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (5V408) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD16/FCGR3 Antibody (5V408) is a Mouse antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Antibody (5V408) can be used in FCM. |
| Alias | Fc receptor, IgG, low affinity III, CD16 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5V408 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human CD16 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD16, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Application | FCM |
| Recommended Dose | FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16b/Fc gamma RIIIb (rh CD16b/Fc gamma RIIIb; TMPY-00701; O75015.2; Met1-Ser200). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
| Conjucates | Unconjugated |
| Others Formats | PerCP/FITC |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CD16b/Fc gamma RIIIb Protein (TMPY-00701) |
| Antigen Species | Human |
| Biology Area | Fc Receptors |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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