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Anti-CD15/SSEA1 Antibody (9S370)

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Catalog No. TMAB-03857

Anti-CD15/SSEA1 Antibody (9S370) is a Rabbit antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (9S370) can be used in FCM, IF, IHC-Fr, IHC-P.

Anti-CD15/SSEA1 Antibody (9S370)

Anti-CD15/SSEA1 Antibody (9S370)

Copy Product Info
😃Good
Catalog No. TMAB-03857
Anti-CD15/SSEA1 Antibody (9S370) is a Rabbit antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (9S370) can be used in FCM, IF, IHC-Fr, IHC-P.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD15/SSEA1 Antibody (9S370) is a Rabbit antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (9S370) can be used in FCM, IF, IHC-Fr, IHC-P.
Ig Type
IgG
Clone
9S370
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03857) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. scatter diagram showing human peripheral blood stained with TMAB-03857. The cells were incubated with the antibody (TMAB-03857, 1: 100) for 30 min at 22°C. Acquisition of >10,000 events was performed.
Application
Recommended Dose
FCM=1 μg/Test; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
Tissue SpecificityHighest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Concentration1mg/ml
Research BackgroundThe Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq].
Antigen Details
Antigen Species
Human
Gene Name
FUT4
Gene ID
Protein Name
alpha-(1, 3)-fucosyltransferase 4
Uniprot ID
Function
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Chemical Properties
Stability & Storage
TransportShipping with blue ice.

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