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Anti-CD15/SSEA1 Antibody (3F592)
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Catalog No. TMAB-03856
Anti-CD15/SSEA1 Antibody (3F592) is a Mouse antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (3F592) can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF.
Anti-CD15/SSEA1 Antibody (3F592)
Anti-CD15/SSEA1 Antibody (3F592)
Copy Product Info😃Good
Catalog No. TMAB-03856
Anti-CD15/SSEA1 Antibody (3F592) is a Mouse antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (3F592) can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CD15/SSEA1 Antibody (3F592) is a Mouse antibody targeting CD15/SSEA1. Anti-CD15/SSEA1 Antibody (3F592) can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF. |
| Ig Type | IgM/Kappa |
| Clone | 3F592 |
| Reactivity | Human,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15/SSEA1 Monoclonal Antibody, Unconjugated (TMAB-03856) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:100-200; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. |
| Tissue Specificity | Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human CD15 |
| Antigen Species | Human |
| Gene Name | FUT4 |
| Gene ID | |
| Protein Name | alpha-(1, 3)-fucosyltransferase 4 |
| Uniprot ID | |
| Function | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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