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Anti-CD14 Antibody (9J346) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (9J346) can be used in FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $270 | 7-10 days |
Description | Anti-CD14 Antibody (9J346) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (9J346) can be used in FCM. |
Alias | CD14 molecule, CD14 |
Ig Type | Monoclonal Rabbit IgG |
Clone | 9J346 |
Reactivity | Mouse |
Verified Activity | 1. Immunofluorescence staining of Mouse CD14 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD14 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane. 2. Flow cytometric analysis of Mouse CD14 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse CD14, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
Application | FCM |
Recommended Dose | FCM: 1:25-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 (rM CD14; TMPY-01225; NP_033971.1; Met1-Pro345). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns. |
Conjucates | Unconjugated |
Others Formats | APC/PerCP/FITC/PE |
Antibody Types Available | 5 |
Immunogen | Recombinant Mouse CD14 protein (TMPY-01225) |
Antigen Species | Mouse |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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