Anti-CD14 Antibody (7X992) is a Mouse antibody targeting CD14. Anti-CD14 Antibody (7X992) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

7X992

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. 1% SDS hot lysates, 25 ug total protein per lane of various lysates (see on figure) probed with CD14 monoclonal antibody, unconjugated (TMAB-03847) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (TMAB-03847) at 1:400 overnight at 4°C, followed by conjugation to the HRP and DAB staining.

IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-2000

Monoclonal

Recombinant Protein: human CD14 protein

Human

CD14

Monocyte differentiation antigen CD14

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.