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Anti-CD14 Antibody (7E908) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (7E908) can be used in ICC,IHC,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-CD14 Antibody (7E908) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (7E908) can be used in ICC,IHC,WB. |
Synonyms | monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen |
Ig Type | IgG |
Clone | 7E908 |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of CD14 on different lysates using anti-CD14 antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver, Lane 2: SW480. 2. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin. 7. ICC staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 55-65 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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