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Anti-CD133/PROM1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00332

Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in WB,FCM.

Anti-CD133/PROM1 Polyclonal Antibody

Anti-CD133/PROM1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00332
Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Fruit Fly)
Verified Activity
1. Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD133 antibody (TMAB-00332), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
2. Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 2: LOVO (Human) Cell Lysate at 30 μg
Lane 3: SW480 (Human) Cell Lysate at 30 μg
Primary: Anti-CD133 antigen (TMAB-00332) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 120 kDa
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell projection, cilium, photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD133
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Neural Stem Cell marker,Surface Molecules,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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