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Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in WB,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Fruit Fly) |
Verified Activity | 1. Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD133 antibody (TMAB-00332), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test. 2. Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 2: LOVO (Human) Cell Lysate at 30 μg Lane 3: SW480 (Human) Cell Lysate at 30 μg Primary: Anti-CD133 antigen (TMAB-00332) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kDa Observed band size: 120 kDa |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell projection, cilium, photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Immunogen | KLH conjugated synthetic peptide: human CD133 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Neural Stem Cell marker,Surface Molecules,Surface Molecules |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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