Anti-CD133/PROM1 Polyclonal Antibody is a Rabbit antibody targeting CD133/PROM1. Anti-CD133/PROM1 Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse (predicted:Rat,Fruit Fly)

1. Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD133 antibody (TMAB-00332), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
2. Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 2: LOVO (Human) Cell Lysate at 30 μg
Lane 3: SW480 (Human) Cell Lysate at 30 μg
Primary: Anti-CD133 antigen (TMAB-00332) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 120 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD133

Human

PROM1

Prominin-1

Neural Stem Cell marker,Surface Molecules,Surface Molecules

Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.