Anti-CD11a Antibody (6I754) is a Mouse antibody targeting CD11a. Anti-CD11a Antibody (6I754) can be used in ICC/IF,FCM.

IgG

6I754

Human

1. Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03824
2. Blank control: Raji.
Primary Antibody (green line): Mouse Anti-CD11a antibody (TMAB-03824)
Dilution: 1:100;
Secondary Antibody: Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

ICC/IF: 1:100-500; FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human CD11a

Human

ITGAL

Integrin alpha-L

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.