Anti-CD10 Antibody (9B971)

Anti-CD10 Antibody (9B971) is a Rabbit antibody targeting CD10. Anti-CD10 Antibody (9B971) can be used in WB,FCM,ICC/IF.

IgG

9B971

Human,Mouse,Rat

1. 4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CD10) monoclonal Antibody, unconjugated (TMAB-03812) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Raji (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD10 antibody (TMAB-03812): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 μg total protein per lane of various lysates (see on figure) probed with CD10 monoclonal antibody, unconjugated (TMAB-03812) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-2000; FCM: 1μg/Test; ICC/IF: 1:100-200

Monoclonal

A synthesized peptide: human Neprilysin

Human

MME

Neprilysin

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.