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Anti-CD10 Antibody (4X679)

Catalog No. TMAB-03813 Copy Product Info
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Anti-CD10 Antibody (4X679) is a Rabbit antibody targeting CD10. Anti-CD10 Antibody (4X679) can be used in WB,IHC-P,IHC-Fr,IP,IF.

Anti-CD10 Antibody (4X679)

Copy Product Info
🥰Excellent
Catalog No. TMAB-03813

Anti-CD10 Antibody (4X679) is a Rabbit antibody targeting CD10. Anti-CD10 Antibody (4X679) can be used in WB,IHC-P,IHC-Fr,IP,IF.

Anti-CD10 Antibody
(4X679)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1517-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CD10 Antibody (4X679) is a Rabbit antibody targeting CD10. Anti-CD10 Antibody (4X679) can be used in WB,IHC-P,IHC-Fr,IP,IF.
Ig Type
IgG
Clone
4X679
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with CD10 monoclonal antibody, unconjugated (TMAB-03813) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated (TMAB-03813) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type II membrane protein.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond.
Antigen Details
Immunogen
A synthesized peptide: human Neprilysin
Antigen Species
Human
Gene Name
MME
Gene ID
Protein Name
Neprilysin
Uniprot ID
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Chemical Properties
Molecular WeightTheoretical: 86 kDa. Actual: 100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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