Anti-CCR7 Antibody (7D23) is a Rabbit antibody targeting CCR7. Anti-CCR7 Antibody (7D23) can be used in WB,ICC/IF,FCM.

IgG

7D23

Human,Mouse,Rat

1. Blank control（black line）:Jurkat.
Primary Antibody (green line): Rabbit Anti-CCR7 antibody (TMAB-03808)
Dilution: 1 ug/Test;
Secondary Antibody（white blue line）: Goat anti-Rabit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. 25 ug total protein per lane of various lysates (see on figure) probed with CCR7 monoclonal antibody, unconjugated (TMAB-03808) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CCR7) monoclonal Antibody, unconjugated (TMAB-03808) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human CCR7

Human

CCR7

C-C chemokine receptor type 7

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.