Anti-CCR2/CD192 Polyclonal Antibody 2 is a Rabbit antibody targeting CCR2/CD192. Anti-CCR2/CD192 Polyclonal Antibody 2 can be used in FCM,IF,IHC-Fr,IHC-P.

IgG

Human,Mouse,Rat

1. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (TMAB-00323)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCR-2) Polyclonal Antibody, Unconjugated (TMAB-00323) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CCR-2

Mouse

CCR2

C-C chemokine receptor type 2

GPCR,Chemokines,Beta Chemokine Rec. (CCR),Macrophage / Inflamm.,Host Virus Interaction,GPCR,STATs

Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.