Anti-CCR2/CD192 Polyclonal Antibody

Anti-CCR2/CD192 Polyclonal Antibody is a Rabbit antibody targeting CCR2/CD192. Anti-CCR2/CD192 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P.

IgG

Human (predicted:Mouse,Rat,Dog,Horse)

1. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (TMAB-00322)
Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 80% methanol for 5 min at-20°C (The cells were fixed with 2% paraformaldehyde for 10 min at room temperature). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CCR2 Polyclonal Antibody, Unconjugated (TMAB-00322) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (TMAB-00322)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CCR-2

Human

CCR2

C-C chemokine receptor type 2

GPCR,Chemokines,Beta Chemokine Rec. (CCR),Macrophage / Inflamm.,Host Virus Interaction,GPCR,STATs

Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.