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Anti-CCR2/CD192 Polyclonal Antibody

😃Good
Catalog No. TMAB-00322
Alias PCLUD, MCP-1-R, FLJ78302, CMKBR2, CKR2B, CKR2A, CKR2, CD192, CCR2B, CCR2A, CCR-2, CC-CKR-2

Anti-CCR2/CD192 Polyclonal Antibody is a Rabbit antibody targeting CCR2/CD192. Anti-CCR2/CD192 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-CCR2/CD192 Polyclonal Antibody

Anti-CCR2/CD192 Polyclonal Antibody

😃Good
Catalog No. TMAB-00322Alias PCLUD, MCP-1-R, FLJ78302, CMKBR2, CKR2B, CKR2A, CKR2, CD192, CCR2B, CCR2A, CCR-2, CC-CKR-2
Anti-CCR2/CD192 Polyclonal Antibody is a Rabbit antibody targeting CCR2/CD192. Anti-CCR2/CD192 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-CCR2/CD192 Polyclonal Antibody is a Rabbit antibody targeting CCR2/CD192. Anti-CCR2/CD192 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
AliasPCLUD, MCP-1-R, FLJ78302, CMKBR2, CKR2B, CKR2A, CKR2, CD192, CCR2B, CCR2A, CCR-2, CC-CKR-2
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Horse)
Verified Activity
1. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (TMAB-00322)
Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 80% methanol for 5 min at-20°C (The cells were fixed with 2% paraformaldehyde for 10 min at room temperature). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CCR2 Polyclonal Antibody, Unconjugated (TMAB-00322) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (TMAB-00322)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CCR-2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
GPCR,Chemokines,Beta Chemokine Rec. (CCR),Macrophage / Inflamm.,Host Virus Interaction,GPCR,STATs
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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