Anti-CCNG2 Polyclonal Antibody is a Rabbit antibody targeting CCNG2. Anti-CCNG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin G2) Polyclonal Antibody, Unconjugated (TMAB-03795) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Brain (Rat) lysate at 30 μg;
Primary: Anti-Cyclin G2 (TMAB-03795) at 1: 200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 38 kD
Observed band size: 38 kD
May be formed of homo-dimer
3. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Cyclin G2 antibody (TMAB-03795)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CCNG2

Human

CCNG2

Cyclin-G2

May play a role in growth regulation and in negative regulation of cell cycle progesssion.