Anti-CCL27 Antibody (5F437) is a Rabbit antibody targeting CCL27. Anti-CCL27 Antibody (5F437) can be used in WB,ICC/IF,FCM.

IgG

5F437

Human,Mouse,Rat

1. Western blot analysis of Human CCL27 recombinant protein cell lysate. Using CCL27 (TMAB-03789) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A431 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CCL27 antibody (TMAB-03789, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human CCL27

Human

CCL27

C-C motif chemokine 27

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.