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Anti-CCL25 Polyclonal Antibody
😃Good
Catalog No. TMAB-00308
Alias Thymus-expressed chemokine, TECK, Small-inducible cytokine A25, SCYA25, Chemokine TECK, CCL25, C-C motif chemokine 25
Anti-CCL25 Polyclonal Antibody is a Rabbit antibody targeting CCL25. Anti-CCL25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CCL25 Polyclonal Antibody
Anti-CCL25 Polyclonal Antibody
😃Good
Catalog No. TMAB-00308Alias Thymus-expressed chemokine, TECK, Small-inducible cytokine A25, SCYA25, Chemokine TECK, CCL25, C-C motif chemokine 25
Anti-CCL25 Polyclonal Antibody is a Rabbit antibody targeting CCL25. Anti-CCL25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CCL25 Polyclonal Antibody is a Rabbit antibody targeting CCL25. Anti-CCL25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | Thymus-expressed chemokine, TECK, Small-inducible cytokine A25, SCYA25, Chemokine TECK, CCL25, C-C motif chemokine 25 |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary at [1:500] for 90 minutes and DAPI staining of the nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary at [1:500] for 90 minutes and DAPI staining of the nucleus. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified. |
| Immunogen | KLH conjugated synthetic peptide: human CCL25 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Beta Chemokines (CC) |
| Molecular Weight | Theoretical: 14 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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