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Anti-CCDC7 Polyclonal Antibody

Catalog No. TMAB-03762 Copy Product Info
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Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CCDC7 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-03762

Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CCDC7 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: Mouse intestine; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CCDC7 Polyclonal Antibody, Unconjugated (TMAB-03762) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC7) Polyclonal Antibody, Unconjugated (TMAB-03762) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC7) Polyclonal Antibody, Unconjugated (TMAB-03762) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Hela (Human) Cell Lysate at 30 μg
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-CCDC7 (TMAB-03762) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. Some proteins that contain coil-coiled domains include c-jun, c-fos and tropomyosin. Coiled-coil domains consist of two or more ?helices packed together via interlacing side chains. CCDC7 (Coiled-coil domain-containing protein 7) is a 486 amino acid protein that contains a coiled-coil domain and is encoded by a gene that maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. There are two isoforms of CCDC7 that are produced as a result of alternative splicing events.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CCDC7
Antigen Species
Human
Gene Name
CCDC7
Gene ID
Protein Name
Coiled-coil domain-containing protein 7
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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