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Anti-CCDC7 Polyclonal Antibody
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Catalog No. TMAB-03762
Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CCDC7 Polyclonal Antibody
Anti-CCDC7 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-03762
Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CCDC7 Polyclonal Antibody is a Rabbit antibody targeting CCDC7. Anti-CCDC7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: Mouse intestine; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CCDC7 Polyclonal Antibody, Unconjugated (TMAB-03762) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC7) Polyclonal Antibody, Unconjugated (TMAB-03762) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC7) Polyclonal Antibody, Unconjugated (TMAB-03762) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Sample: Hela (Human) Cell Lysate at 30 μg Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-CCDC7 (TMAB-03762) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. Some proteins that contain coil-coiled domains include c-jun, c-fos and tropomyosin. Coiled-coil domains consist of two or more ?helices packed together via interlacing side chains. CCDC7 (Coiled-coil domain-containing protein 7) is a 486 amino acid protein that contains a coiled-coil domain and is encoded by a gene that maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. There are two isoforms of CCDC7 that are produced as a result of alternative splicing events. |
| Immunogen | KLH conjugated synthetic peptide: human CCDC7 |
| Antigen Species | Human |
| Gene Name | CCDC7 |
| Gene ID | |
| Protein Name | Coiled-coil domain-containing protein 7 |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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