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Anti-CCDC134 Antibody (8H634)

😃Good
Catalog No. TMAY-02519
Alias coiled-coil domain containing 134

Anti-CCDC134 Antibody (8H634) is a Rabbit antibody targeting CCDC134. Anti-CCDC134 Antibody (8H634) can be used in WB,IP.

Anti-CCDC134 Antibody (8H634)

Anti-CCDC134 Antibody (8H634)

😃Good
Catalog No. TMAY-02519Alias coiled-coil domain containing 134
Anti-CCDC134 Antibody (8H634) is a Rabbit antibody targeting CCDC134. Anti-CCDC134 Antibody (8H634) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: WB, IP

Reactivity: Human
Aliascoiled-coil domain containing 134
Ig Type
Rabbit IgG
Clone
8H634
Reactivity
Human
Verified Activity
1. Anti-CCDC134 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HL-60 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:26 kDa.
-Observed band size:26 kDa.
2. CCDC134 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-CCDC134 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-CCDC134 rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 28 kDa.
-Observed band size: 28 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human CCDC134 (rh CCDC134; TMPY-02551; Q9H6E4; Met1-Leu229).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCoiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CCDC134 protein (TMPY-02551)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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