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Anti-CCDC134 Antibody (8H634) is a Rabbit antibody targeting CCDC134. Anti-CCDC134 Antibody (8H634) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
Alias | coiled-coil domain containing 134 |
Ig Type | Rabbit IgG |
Clone | 8H634 |
Reactivity | Human |
Verified Activity | 1. Anti-CCDC134 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 2. CCDC134 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-CCDC134 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CCDC134 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 28 kDa. -Observed band size: 28 kDa |
Application | WB,IP |
Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CCDC134 (rh CCDC134; TMPY-02551; Q9H6E4; Met1-Leu229). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human CCDC134 protein (TMPY-02551) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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