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Anti-CCDC11 Polyclonal Antibody
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Catalog No. TMAB-03730
Anti-CCDC11 Polyclonal Antibody is a Rabbit antibody targeting CCDC11. Anti-CCDC11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CCDC11 Polyclonal Antibody
Anti-CCDC11 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-03730
Anti-CCDC11 Polyclonal Antibody is a Rabbit antibody targeting CCDC11. Anti-CCDC11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CCDC11 Polyclonal Antibody is a Rabbit antibody targeting CCDC11. Anti-CCDC11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC11) Polyclonal Antibody, Unconjugated (TMAB-03730) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: A431 (Human) Cell Lysate at 30 μg Primary: Anti-CCDC11 (TMAB-03730) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 63 kD 3. 25 μg total protein per lane of various lysates (see on figure) probed with CCDC11 polyclonal antibody, unconjugated (TMAB-03730) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CCDC11 is a 514 amino acid protein encode by a gene that maps to human chromosome 18q21.1. Encoding over 300 genes, chromosome 18 contains about 76 million bases. Trisomy 18, or Edwards syndrome, is the second most common trisomy after Downs syndrome. Symptoms of Edwards syndrome include low birth weight, a variety of physical development defects, heart deformations and breathing difficulty. Translocation between chromosome 18 and 14 is the most common translocation in cancers, and occurs in follicular lymphomas. Niemann-Pick disease, hereditary hemorrhagic telangiectasia and erythropoietic protoporphyria are associated with chromosome 18. The TGF∫ modulators, Smad2, Smad4 and Smad7 are encoded by chromosome 18. |
| Immunogen | KLH conjugated synthetic peptide: human CCDC11 |
| Antigen Species | Human |
| Gene Name | CFAP53 |
| Gene ID | |
| Protein Name | Cilia-and flagella-associated protein 53 |
| Uniprot ID |
| Molecular Weight | Theoretical: 57 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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