Anti-CBX4 Polyclonal Antibody is a Rabbit antibody targeting CBX4. Anti-CBX4 Polyclonal Antibody can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Horse,Sheep)

1. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human K562 cell lysates
Lane 4: Human HL-60 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human HUVEC cell lysates
Lane 8: Human A549 cell lysates
Primary: Anti-CBX4 (TMAB-03721) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (TMAB-03721) 1: 25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; ICC/IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CBX4

Human

CBX4

E3 SUMO-protein ligase CBX4

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.