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Anti-CBX4 Antibody (6H942)

Catalog No. TMAB-03722 Copy Product Info
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Anti-CBX4 Antibody (6H942) is a Rabbit antibody targeting CBX4. Anti-CBX4 Antibody (6H942) can be used in IF, IHC-Fr, IHC-P.

Anti-CBX4 Antibody (6H942)

Copy Product Info
🥰Excellent
Catalog No. TMAB-03722

Anti-CBX4 Antibody (6H942) is a Rabbit antibody targeting CBX4. Anti-CBX4 Antibody (6H942) can be used in IF, IHC-Fr, IHC-P.

Anti-CBX4 Antibody
(6H942)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1517-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4717-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CBX4 Antibody (6H942) is a Rabbit antibody targeting CBX4. Anti-CBX4 Antibody (6H942) can be used in IF, IHC-Fr, IHC-P.
Ig Type
IgG
Clone
6H942
Reactivity
Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CBX4 Monoclonal Antibody, Unconjugated (TMAB-03722) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
Application
Recommended Dose
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityUbiquitous.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEnables SUMO binding activity; SUMO ligase activity; and enzyme binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in nuclear body. Part of PRC1 complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma. [provided by Alliance of Genome Resources, Apr 2022]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CBX4
Antigen Species
Human
Gene Name
CBX4
Gene ID
Protein Name
E3 SUMO-protein ligase CBX4
Uniprot ID
Function
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Chemical Properties
Molecular WeightTheoretical: 61 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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