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Anti-Cbl-c Antibody (8B374) is a Mouse antibody targeting Cbl-c. Anti-Cbl-c Antibody (8B374) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $427 | 7-10 days |
Description | Anti-Cbl-c Antibody (8B374) is a Mouse antibody targeting Cbl-c. Anti-Cbl-c Antibody (8B374) can be used in WB,ICC,IHC,FCM. |
Synonyms | RNF57, CBL-SL, CBL-3, Cbl proto-oncogene C, E3 ubiquitin protein ligase |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1,000 dilution. 2. Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1,000 dilution.Positive control:Lane 1: RAJI, Lane 2: RAW264.7, Lane 3: K562, Lane 4: SKBR-3, Lane 5: 3T3-L1, Lane 6: THP-1, Lane 7: PC-12. 3. Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin. 5. ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:100-200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 120 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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