Anti-Caveolin-3 Polyclonal Antibody 2 is a Rabbit antibody targeting Caveolin-3. Anti-Caveolin-3 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Rabbit)

1. Sample:
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-Caveolin-3 (TMAB-03706) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
2. Blank control (blue line): A431 (blue).
Primary Antibody (green line): Rabbit Anti-Caveolin-3 antibody (TMAB-03706);
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): F (ab’)2 fragment goat anti-rabbit IgG-FITC;Dilution: 1 μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% P then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse Caveolin-3

Mouse

CAV3

Caveolin-3

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.