Anti-Caveolin-2 Polyclonal Antibody is a Rabbit antibody targeting Caveolin-2. Anti-Caveolin-2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Rat (predicted:Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Caveolin-2) Polyclonal Antibody, Unconjugated (TMAB-03704) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Caveolin-2) Polyclonal Antibody, Unconjugated (TMAB-03704) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Caveolin-2) Polyclonal Antibody, Unconjugated (TMAB-03704) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. 4% Paraformaldehyde-fixed HUVEC (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Caveolin-2) polyclonal Antibody, unconjugated (TMAB-03704) 1: 200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Caveolin-2

Human

CAV2

Caveolin-2

May act as a scaffolding protein within caveolarmembranes. Interacts directly with G-protein alpha subunits and canfunctionally regulate their activity. Acts as an accessory proteinin conjunction with CAV1 in targeting to lipid rafts and drivingcaveolae formation. The Ser-36 phosphorylated form has a role inmodulating mitosis in endothelial cells. Positive regulator ofcellular mitogenesis of the MAPK signaling pathway. Required forthe insulin-stimulated nuclear translocation and activation ofMAPK1 and STAT3, and the subsequent regulation of cell cycleprogression (By similarity).