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Anti-CAV1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00299
Alias VIP21, VIP 21, PPH3, OTTHUMP00000025031, MSTP085, LCCNS, CGL3, cell growth-inhibiting protein 32, Caveolin-1, Caveolin1, Caveolin 1 caveolae protein 22kDa, caveolin 1 beta isoform, caveolin 1 alpha isoform, caveolae protein, 22 kD, CAV 1, CAV, BSCL3
Anti-CAV1 Polyclonal Antibody is a Rabbit antibody targeting CAV1. Anti-CAV1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CAV1 Polyclonal Antibody
Anti-CAV1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00299Alias VIP21, VIP 21, PPH3, OTTHUMP00000025031, MSTP085, LCCNS, CGL3, cell growth-inhibiting protein 32, Caveolin-1, Caveolin1, Caveolin 1 caveolae protein 22kDa, caveolin 1 beta isoform, caveolin 1 alpha isoform, caveolae protein, 22 kD, CAV 1, CAV, BSCL3
Anti-CAV1 Polyclonal Antibody is a Rabbit antibody targeting CAV1. Anti-CAV1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CAV1 Polyclonal Antibody is a Rabbit antibody targeting CAV1. Anti-CAV1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | VIP21, VIP 21, PPH3, OTTHUMP00000025031, MSTP085, LCCNS, CGL3, cell growth-inhibiting protein 32, Caveolin-1, Caveolin1, Caveolin 1 caveolae protein 22kDa, caveolin 1 beta isoform, caveolin 1 alpha isoform, caveolae protein, 22 kD, CAV 1, CAV, BSCL3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (TMAB-00299) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. 2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (TMAB-00299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: mouse retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (TMAB-00299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lung (Mouse) Lysate at 40 μg Bladder (Mouse) Lysate at 40 μg Primary: Anti-Caveolin-1 (TMAB-00299) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa 5. Sample: NIH/3T3 Cell Lysate at 40 μg A549 Cell Lysate at 40 μg HUVEC Cell Lysate at 40 μg Primary: Anti-Caveolin-1 (TMAB-00299) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa 6. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-Caveolin-1 (TMAB-00299) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus membrane;Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human Caveolin-1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Tumor biomarkers,Cholesterol Metabolism,Cholesterol Metabolism,Hypoxia,Coat Proteins,Caveolae and Clathrin |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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