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Anti-Catalase Antibody (2V407)

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Catalog No. TMAB-03680 Copy Product Info
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Anti-Catalase Antibody (2V407) is a Mouse antibody targeting Catalase. Anti-Catalase Antibody (2V407) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Catalase Antibody (2V407)
Pack SizePriceUSA StockGlobal StockQuantity
25 µL$1487-10 days7-10 days
50 µL$2637-10 days7-10 days
100 µL$4717-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Catalase Antibody (2V407) is a Mouse antibody targeting Catalase. Anti-Catalase Antibody (2V407) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1, Kappa
Clone
2V407
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human 293T cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Raji cell lysates
Primary: Anti-Catalase (TMAB-03680) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (TMAB-03680) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (TMAB-03680) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (TMAB-03680) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationPeroxisome.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCatalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Catalase
Antigen Species
Human
Gene Name
CAT
Gene ID
Protein Name
Catalase
Uniprot ID
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Chemical Properties
Molecular WeightTheoretical: 60 kDa. Actual: 58 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-Catalase Antibody (2V407) chemical structure | Anti-Catalase Antibody (2V407) molecular weight