Anti-CASPR Polyclonal Antibody is a Rabbit antibody targeting CASPR. Anti-CASPR Polyclonal Antibody can be used in ICC/IF,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse)

1. Tissue/cell: human glioma cells (U251 cells); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated (TMAB-03676) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
2. Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit CASPR antibody (TMAB-03676)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

ICC/IF: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CASPR/Neurexin4

Human

CNTNAP1

Contactin-associated protein 1

Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.