Anti-Caspase-3 Antibody (1Q554) is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Antibody (1Q554) can be used in WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF.

IgG

1Q554

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-03660) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-03660) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-03660) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-03660) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Jurkat (H) cells were treated with or without staurosporine treated (1 uM) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Caspase-3 monoclonal antibody, unconjugated (TMAB-03660) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
6. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Caspase-3) monoclonal Antibody, unconjugated (TMAB-03660) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
7. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-Caspase-3 antibody (TMAB-03660): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-5000; IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800; FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: human Caspase-3 protein

Human

CASP3

Caspase-3

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.