Anti-Caspase-1 p20 Polyclonal Antibody 3 is a Rabbit antibody targeting Caspase-1 p20. Anti-Caspase-1 p20 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Blank control: HL-60.
Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (TMAB-03650)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Thymus tissue lysates
Lane 3: Human THP-1 cell lysates
Lane 4: Human U937 cell lysates
Primary: Anti-Caspase-1 p20 (TMAB-03650) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/46 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Caspase-1 p20

Human

CASP1

Caspase-1

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.