Anti-Caspase-1 P10 Polyclonal Antibody 3 is a Rabbit antibody targeting Caspase-1 P10. Anti-Caspase-1 P10 Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Human,Mouse

1. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-Caspase-1 P10 (TMAB-03649) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 45 kD
2. Sample:
Lung Cell (Mouse) Lysate at 40 μg
Primary: Anti-NKG2D (TMAB-03649) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 45 kD
3. Blank control: HL-60.
Primary Antibody (green line): Rabbit Anti-Caspase-1 P10 antibody (TMAB-03649)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-se-1 P10 antibody (TMAB-03649), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg /test.
5. Sample:
Liver Cell (Mouse) Lysate at 40 μg
Primary: Anti-NKG2D (TMAB-03649) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 45 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Caspase-1 P10

Human

CASP1

Caspase-1

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.