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Anti-Caspase-1 p10 Polyclonal Antibody 2
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Catalog No. TMAB-00281
Anti-Caspase-1 p10 Polyclonal Antibody 2 is a Rabbit antibody targeting Caspase-1 p10. Anti-Caspase-1 p10 Polyclonal Antibody 2 can be used in WB,FCM.
Anti-Caspase-1 p10 Polyclonal Antibody 2
Anti-Caspase-1 p10 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00281
Anti-Caspase-1 p10 Polyclonal Antibody 2 is a Rabbit antibody targeting Caspase-1 p10. Anti-Caspase-1 p10 Polyclonal Antibody 2 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Rat (predicted:Mouse) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-Caspase-1 P10 antibody (TMAB-00281) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1: Small intestine (Rat) Lysate at 40 μg Lane 2: Urinary bladder (Rat) Lysate at 40 μg Primary: Anti-Caspase-1 P10 (TMAB-00281) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/10 kDa Observed band size: 45/10 kDa |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: mouse Caspase-1 P10 |
| Antigen Species | Mouse |
| Gene ID | 12362 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Caspases,Metabolism,Caspases,Caspases,Apoptosis,Hypoxia |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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