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Anti-CASP9 Antibody (5N183) is a Mouse antibody targeting CASP9. Anti-CASP9 Antibody (5N183) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $218 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-CASP9 Antibody (5N183) is a Mouse antibody targeting CASP9. Anti-CASP9 Antibody (5N183) can be used in WB,IP. |
Synonyms | MCH6, ICE-like apoptotic protease 6, ICE-LAP6, EC 3.4.22.62, Caspase-9, Caspase9, CASP-9, Apoptotic protease-activating factor 3, Apoptotic protease Mch-6, APAF-3 |
Clone | 5N183 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Hela cells, using TMAC-00574 diluted at 1:2,000 1:5,000. 2. Western blot analysis of 1)Jurkat, 2) HepG2, 3)Mouse Heart, 4)Rat Heart, using TMAC-00574 diluted at 1:3,000. 3. Input: Hela Cell Lysate, IP product: IP dilute 1:200. Western blot analysis: primary antibody : TMAC-00574 1:1,000, Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000. |
Application | |
Recommended Dose | WB: 1:1000-5000;IP: 1:200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Affinity purification using immunogen. |
Appearance | Liquid |
Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
Conjucates | Unconjugated |
Uniprot ID |
Molecular Weight | Actual: 46 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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