Anti-Cardiac Troponin I/TNIC Antibody (5E33) is a Mouse antibody targeting Cardiac Troponin I/TNIC. Anti-Cardiac Troponin I/TNIC Antibody (5E33) can be used in IHC-P,IHC-Fr,IF.

IgG

5E33

Human,Rat,Mouse

1. Negative sample: Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Negative sample: Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Negative sample: Paraformaldehyde-fixed, paraffin embedded (mouse brain);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat brain);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (TMAB-03628) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human Cardiac Troponin I (cTnI)

Human

TNNI3

Troponin I, cardiac muscle

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.