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Anti-Cardiac Troponin I (cTnI) Antibody (5I456)

Catalog No. TMAB-03627 Copy Product Info
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Anti-Cardiac Troponin I (cTnI) Antibody (5I456) is a Mouse antibody targeting Cardiac Troponin I (cTnI). Anti-Cardiac Troponin I (cTnI) Antibody (5I456) can be used in IHC-P,IHC-Fr,IF.

Anti-Cardiac Troponin I (cTnI) Antibody (5I456)

Copy Product Info
🥰Excellent
Catalog No. TMAB-03627

Anti-Cardiac Troponin I (cTnI) Antibody (5I456) is a Mouse antibody targeting Cardiac Troponin I (cTnI). Anti-Cardiac Troponin I (cTnI) Antibody (5I456) can be used in IHC-P,IHC-Fr,IF.

Anti-Cardiac Troponin I
(cTnI) Antibody
(5I456)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$3547-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Cardiac Troponin I (cTnI) Antibody (5I456) is a Mouse antibody targeting Cardiac Troponin I (cTnI). Anti-Cardiac Troponin I (cTnI) Antibody (5I456) can be used in IHC-P,IHC-Fr,IF.
Clone
5I456
Reactivity
Human
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cardiac Troponin I (cTnI)) Monoclonal Antibody, Unconjugated (TMAB-03627) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:200-2000; IHC-Fr: 1:200-2000; IF: 1:200-2000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundTroponin I is a cardiac and skeletal muscle protein useful in the laboratory diagnosis of heart attack. Troponin I is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CTn1
Antigen Species
Human
Gene Name
TNNI3
Gene ID
Protein Name
Troponin I, cardiac muscle
Uniprot ID
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Chemical Properties
Molecular WeightTheoretical: 23 kDa. Actual: 34-35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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