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Anti-CARD14 Polyclonal Antibody
Catalog No. TMAB-03625 Copy Product Info
🥰Excellent
Anti-CARD14 Polyclonal Antibody is a Rabbit antibody targeting CARD14. Anti-CARD14 Polyclonal Antibody can be used in WB,ICC/IF.
Anti-CARD14 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-03625
Anti-CARD14 Polyclonal Antibody is a Rabbit antibody targeting CARD14. Anti-CARD14 Polyclonal Antibody can be used in WB,ICC/IF.
Anti-CARD14 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-CARD14 Polyclonal Antibody is a Rabbit antibody targeting CARD14. Anti-CARD14 Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CARD14) polyclonal Antibody, Unconjugated (TMAB-03625) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. 2. Sample: Jurkat (Human) Cell Lysate at 30 μg 293T (Human) Cell Lysate at 30 μg Primary: Anti-CARD14 (TMAB-03625) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD 3. Sample: Fetal brain (Mouse) Lysate at 40 μg Primary: Anti-CARD14 (TMAB-03625) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD 4. Sample: Placenta (Mouse) Lysate at 40 μg Primary: Anti-CARD14 (TMAB-03625) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cytoplasm.Isoform 2: Cytoplasm.Isoform 3: Cytoplasm. |
| Tissue Specificity | Isoform 1 is detected in placenta and epidermal keratinocytes. Isoform 2 is detected in leukocytes and fetal brain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein shares a similar domain structure with CARD11 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. Two alternatively spliced variants of this gene encoding distinct isoforms have been reported. |
| Immunogen | KLH conjugated synthetic peptide: human CARD14 |
| Antigen Species | Human |
| Gene Name | CARD14 |
| Gene ID | |
| Protein Name | Caspase recruitment domain-containing protein 14 |
| Uniprot ID | |
| Function | Plays a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. |
| Molecular Weight | Theoretical: 113 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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