Anti-CARD11 Antibody (9H360) is a Rabbit antibody targeting CARD11. Anti-CARD11 Antibody (9H360) can be used in WB,ICC/IF,FCM.

IgG

9H360

Human,Mouse

1. 25 μg total protein per lane of various lysates (see on figure) probed with CARD11 monoclonal antibody, unconjugated (TMAB-03623) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CARD11 antibody (TMAB-03623, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human CARD11

Human

CARD11

Caspase recruitment domain-containing protein 11

Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.