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Anti-Carbonic Anhydrase 8 Antibody (6D426)

😃Good
Catalog No. TMAY-00873
Alias wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993

Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP.

Anti-Carbonic Anhydrase 8 Antibody (6D426)

Anti-Carbonic Anhydrase 8 Antibody (6D426)

😃Good
Catalog No. TMAY-00873Alias wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP.
Aliaswdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Ig Type
Monoclonal Rabbit IgG
Clone
6D426
Reactivity
Human
Verified Activity
1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:33 kDa.

-Observed band size:38 kDa.

2. CA8 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg A549 Whole Cell Lysate

-0.5 µL anti-CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-CA8 rabbit monoclonal antibody, at 1:150 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 37 kDa.

-Observed band size: 37 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; TMPY-01706; NP_004047.3; Met1-Gln290).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human Carbonic Anhydrase VIII / CA8 protein (TMPY-01706)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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