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Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $268 | 7-10 days |
Description | Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP. |
Alias | wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993 |
Ig Type | Monoclonal Rabbit IgG |
Clone | 6D426 |
Reactivity | Human |
Verified Activity | 1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:38 kDa. 2. CA8 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg A549 Whole Cell Lysate -0.5 µL anti-CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CA8 rabbit monoclonal antibody, at 1:150 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 37 kDa. -Observed band size: 37 kDa |
Application | WB,IP |
Recommended Dose | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; TMPY-01706; NP_004047.3; Met1-Gln290). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human Carbonic Anhydrase VIII / CA8 protein (TMPY-01706) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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