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Anti-Carbonic Anhydrase 8 Antibody (2N156)

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Catalog No. TMAY-03451

Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP.

Anti-Carbonic Anhydrase 8 Antibody (2N156)

Anti-Carbonic Anhydrase 8 Antibody (2N156)

😃Good
Catalog No. TMAY-03451
Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP.
Ig Type
Monoclonal Rabbit IgG
Clone
2N156
Reactivity
Mouse
Verified Activity
1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: 293T Whole Cell Lysate.

-Lane B: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:33 kDa.

-Observed band size:36 kDa.

2. Mouse CA8 was immunoprecipitated using:

-Lane A:0.5 mg 293T Whole Cell Lysate.

-Lane B:0.5 mg A549 Whole Cell Lysate.

-2 µL anti-Mouse CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-Mouse CA8 rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 33 kDa.

-Observed band size: 37 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rM Carbonic Anhydrase VIII / CA8; TMPY-02301; P28651; Met1-Gln291).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Mouse Carbonic Anhydrase VIII / CA8 protein (TMPY-02301)
Antigen Species
Mouse
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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