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Anti-Carbonic Anhydrase 8 Antibody (2N156)
😃Good
Catalog No. TMAY-03451
Alias wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP.
Anti-Carbonic Anhydrase 8 Antibody (2N156)
Anti-Carbonic Anhydrase 8 Antibody (2N156)
😃Good
Catalog No. TMAY-03451Alias wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Mouse |
| Alias | wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993 |
| Ig Type | Rabbit IgG |
| Clone | 2N156 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:36 kDa. 2. Mouse CA8 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg A549 Whole Cell Lysate. -2 µL anti-Mouse CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse CA8 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 37 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rM Carbonic Anhydrase VIII / CA8; TMPY-02301; P28651; Met1-Gln291). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Mouse Carbonic Anhydrase VIII / CA8 protein (TMPY-02301) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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