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Anti-Carbonic Anhydrase 3 Antibody (8M461)
😃Good
Catalog No. TMAY-00912
Alias carbonic anhydrase III, Car3, CAIII
Anti-Carbonic Anhydrase 3 Antibody (8M461) is a Rabbit antibody targeting Carbonic Anhydrase 3. Anti-Carbonic Anhydrase 3 Antibody (8M461) can be used in WB,IP.
Anti-Carbonic Anhydrase 3 Antibody (8M461)
Anti-Carbonic Anhydrase 3 Antibody (8M461)
😃Good
Catalog No. TMAY-00912Alias carbonic anhydrase III, Car3, CAIII
Anti-Carbonic Anhydrase 3 Antibody (8M461) is a Rabbit antibody targeting Carbonic Anhydrase 3. Anti-Carbonic Anhydrase 3 Antibody (8M461) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Carbonic Anhydrase 3 Antibody (8M461) is a Rabbit antibody targeting Carbonic Anhydrase 3. Anti-Carbonic Anhydrase 3 Antibody (8M461) can be used in WB,IP. |
| Alias | carbonic anhydrase III, Car3, CAIII |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8M461 |
| Reactivity | Human |
| Verified Activity | 1. Anti-CA3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:30 kDa. -Observed band size:34 kDa. 2. CA3 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-CA3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CA3 rabbit monoclonal antibody, at 1:250 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 30 kDa. -Observed band size: 30 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; TMPY-01761; NP_005172.1; Met1-Lys260). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Carbonic Anhydrase III / CA3 protein (TMPY-01761) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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