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Anti-Carbonic Anhydrase 2 Antibody (8K348)
😃Good
Catalog No. TMAY-00879
Alias Lvtw-5, Ltw-5, carbonic anhydrase II, Car-2, CAII, Ca2, AI131712
Anti-Carbonic Anhydrase 2 Antibody (8K348) is a Rabbit antibody targeting Carbonic Anhydrase 2. Anti-Carbonic Anhydrase 2 Antibody (8K348) can be used in WB,IP.
Anti-Carbonic Anhydrase 2 Antibody (8K348)
Anti-Carbonic Anhydrase 2 Antibody (8K348)
😃Good
Catalog No. TMAY-00879Alias Lvtw-5, Ltw-5, carbonic anhydrase II, Car-2, CAII, Ca2, AI131712
Anti-Carbonic Anhydrase 2 Antibody (8K348) is a Rabbit antibody targeting Carbonic Anhydrase 2. Anti-Carbonic Anhydrase 2 Antibody (8K348) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | Lvtw-5, Ltw-5, carbonic anhydrase II, Car-2, CAII, Ca2, AI131712 |
| Ig Type | Rabbit IgG |
| Clone | 8K348 |
| Reactivity | Human |
| Verified Activity | 1. Anti-CA2 rabbit monoclonal antibody at 1:10000 dilution. -Lane A: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:30 kDa. 2. CA2 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -2 µL anti-CA2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CA2 rabbit monoclonal antibody, at 1:2000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 29 kDa. -Observed band size: 29 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:10000-1:50000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase II / CA2 (rh Carbonic Anhydrase II / CA2; TMPY-01735; NP_000058.1; Met1-Lys260). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Carbonic Anhydrase II / CA2 protein (TMPY-01735) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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