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Anti-CAPN2 Antibody (6Z187) is a Rabbit antibody targeting CAPN2. Anti-CAPN2 Antibody (6Z187) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-CAPN2 Antibody (6Z187) is a Rabbit antibody targeting CAPN2. Anti-CAPN2 Antibody (6Z187) can be used in WB,ICC,IHC,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin. 4. ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 80 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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