Anti-CAPN1 Antibody (6P683) is a Mouse antibody targeting CAPN1. Anti-CAPN1 Antibody (6P683) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG1, k

6P683

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human kidney section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CAPN1) Monoclonal Antibody, Unconjugated (TMAB-03609) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Blank control: Hela.
Primary Antibody (green line): Mouse Anti-CAPN1 antibody (TMAB-03609)
Dilution: 1: 25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody: Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample:
Lane 1: A431 cell lysates
Lane 2: A549 cell lysates
Lane 3: MCF-7 cell lysates
Lane 4: U-251 MG cell lysates
Primary: Anti-CAPN1 (TMAB-03609) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 110 kD

WB: 1:500-2000; IHC-P: 1:25-50; IHC-Fr: 1:25-100; IF: 1:100-500; FCM: 1:50-100

Monoclonal

KLH conjugated synthetic peptide: human CAPN1

Human

CAPN1

Calpain-1 catalytic subunit

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.