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Anti-CAMKK2 Polyclonal Antibody

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Catalog No. TMAB-00276

Anti-CAMKK2 Polyclonal Antibody is a Rabbit antibody targeting CAMKK2. Anti-CAMKK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-CAMKK2 Polyclonal Antibody

Anti-CAMKK2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00276
Anti-CAMKK2 Polyclonal Antibody is a Rabbit antibody targeting CAMKK2. Anti-CAMKK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CAMKK2 Polyclonal Antibody is a Rabbit antibody targeting CAMKK2. Anti-CAMKK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Pig,Cow,Horse,Sheep)
Verified Activity
1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-CAMKK2 antibody (TMAB-00276)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (CAMKK2) Polyclonal Antibody, Unconjugated (TMAB-00276) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Molt-4 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CAMKK2 Antibody (TMAB-00276) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
4. Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human MOLT4 cell lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-CAMKK2 (TMAB-00276) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells. Found in the cytoplasm and neurites after forskolin induction.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CAMKK2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
MAPK pathway,MAPK Pathway,Calmodulin Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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