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Anti-CAMKIV Antibody (5K734)
😃Good
Catalog No. TMAC-00550
Alias D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik
Anti-CAMKIV Antibody (5K734) is a Rabbit antibody targeting CAMKIV. Anti-CAMKIV Antibody (5K734) can be used in FCM,ICC,IF,IHC,WB.
Anti-CAMKIV Antibody (5K734)
Anti-CAMKIV Antibody (5K734)
😃Good
Catalog No. TMAC-00550Alias D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik
Anti-CAMKIV Antibody (5K734) is a Rabbit antibody targeting CAMKIV. Anti-CAMKIV Antibody (5K734) can be used in FCM,ICC,IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-CAMKIV Antibody (5K734) is a Rabbit antibody targeting CAMKIV. Anti-CAMKIV Antibody (5K734) can be used in FCM,ICC,IF,IHC,WB. |
| Synonyms | D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik |
| Ig Type | IgG |
| Clone | 5K734 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 5. ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: C-terminus human CAMKIV |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 52 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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